Wednesday, 25 March 2015

The coding gene downstream of the cloned promoter encodes luciferase reporter gene (Luc)

The coding gene downstream of the cloned promoter encodes luciferase reporter gene (Luc).
: The figure below shows you a series of deletion mutations made to a promoter sequence which cloned into a pLG3 reporter plasmid vector. The coding gene downstream of the cloned promoter encodes luciferase reporter gene (Luc). Explain these data using a numbered list from 1 10 with 1 being the SV40 wild type viral promoter construct and going down the constructs in order. Why some promoter construct results high luciferase activity and some does not?
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